Table 1 The primers used in RT–qPCR.

From: The asymmetrical ROS–METTL3–ESR1 axis in paraspinal muscle progenitor cells determines the progression of adolescent idiopathic scoliosis

Primer name

Forward (5′–3′)

Reverse (5′–3′)

Human GAPDH

CAAGGCTGAGAACGGGAAGC

AGGGGGCAGAGATGATGACC

Human METTL3

TTGTCTCCAACCTTCCGTAGT

CCAGATCAGAGAGGTGGTGTAG

Human ESR1

CCCACTCAACAGCGTGTCTC

CGTCGATTATCTGAATTTGGCCT

Human MYH1

GGGAGACCTAAAATTGGCTCAA

TTGCAGACCGCTCATTTCAAA

Human MYH3

ATTGCTTCGTGGTGGACTCAA

GGCCATGTCTTCGATCCTGTC

Human CKM

ATGCCATTCGGTAACACCCAC

GCTTCTTGTAGAGTTCAAGGGTC

Human MYOG

GGGGAAAACTACCTGCCTGTC

AGGCGCTCGATGTACTGGAT

Mouse Gapdh

ACCCAGAAGACTGTGGATGG

ACACATTGGGGGTAGGAACA

Mouse Esr1

CCCGCCTTCTACAGGTCTAAT

CTTTCTCGTTACTGCTGGACAG

Mouse Myh1

GCGAATCGAGGCTCAGAACAA

GTAGTTCCGCCTTCGGTCTTG

Mouse Myh3

ATGAGTAGCGACACCGAGATG

ACAAAGCAGTAGGTTTTGGCAT

Mouse Ckm

GGCAACACCCACAACAAGTTC

CCTTGAAGACCGTGTAGGACT　

Mouse MyoG

GAGACATCCCCCTATTTCTACCA

GCTCAGTCCGCTCATAGCC

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