Fig. 2: Modeling DAE in the developing mouse brain.
From: Reversibility and therapeutic feasibility of DNM1L-associated neurodevelopmental disorders
a Scheme of DAE mouse model generation via IUE. b Representative images of brain slices electroporated with each variant and stained with anti-GFP. Green, GFP; blue, DAPI. Scale bar, 1 mm. c, d Representative confocal z-stack images and quantification of 35-µm sections of a P21 mouse brain CC immunostained with anti-GFP. Green, GFP; blue, DAPI. Scale bar, 100 μm. Control, n = 7; wild type, n = 4; G350R, n = 4; L416P, n = 4; L650R, n = 5. e Representative images of P21 ipsilateral and contralateral cortical plates electroporated with each variant. Slices were immunostained with anti-GFP. Black, GFP. Scale bar, 200 μm. f Quantification of P21 ipsilateral cortical plates electroporated with each variant. Control, n = 7; wild type, n = 4; G350R, n = 4; L416P, n = 3; L650R, n = 5. g Quantification of P21 contralateral cortical plates electroporated with each variant. Control, n = 7; wild type, n = 4; G350R, n = 4; L416P, n = 3; L650R, n = 5. Bar plot indicates mean ± s.e.m. Statistical significance is determined by the Kruskal–Wallis test with Dunn’s post hoc test for d and two-way ANOVA with Bonferroni’s post hoc test for f and g. *P < 0.05; **P < 0.01; ****P < 0.0001. Panel a created with BioRender.com.
