Fig. 2: Psrc1 has an effect on Trp metabolism in atherosclerosis.

a The relative mRNA levels of essential Trp metabolic enzymes (to β-actin) in liver were determined using RT–qPCR (n = 6). b The Trp metabolites in plasma (n = 6). c,d The relative mRNA levels of Ahr, Cyp1a1 and Cyp1b1 (to β-actin) in liver (c) and colon (d) were determined using RT–qPCR (n = 6). e Immunofluorescence of aortic root cross-sections demonstrating staining for Ahr in plaques. Scale bar, 200 μm. f Psrc1 protein expression in Raw264.7, MOVAS and MAEC determined by western blotting. g Immunofluorescence of aortic root cross-sections demonstrating staining for Psrc1 in CD68⁺ macrophages and α-SMA⁺ smooth muscle cells. Scale bar, 150 μm. h Representative images of immunofluorescence staining of Ahr protein and DAPI for nuclei in MPM isolated from Psrc1^+/+Apoe^−/− and Psrc1^−/−Apoe^−/− mice. Scale bar, 50 μm (top) and 10 μm (bottom). Data are presented as mean ± s.d. Statistical analysis was performed by two-tailed unpaired t-tests (Ido1, Ido2, Kynu, Kmo, Mao, Kat1, Kat2 and Kat3 in a and b and Ahr in c and d) and Mann–Whitney U test (Tdo and Tph1 in a and Cyp1a1 and Cyp1b1 in c).