Fig. 2: Liver-specific KO of Ythdf1 predisposes mice to MASLD.

a Representative images of liver from HFD-fed WT and Ythdf1-KO mice. n = 6, respectively. b Quantitative analysis of liver weight and liver-to-body weight ratio in HFD-fed WT and Ythdf1-KO mice. n = 6. c Representative images of H&E staining and representative images of Oil Red O staining. n = 6. d Quantification of the MASH scores of hepatic tissues from HFD-fed WT and Ythdf1-KO mice. n = 6. Quantification of the Oil Red O area of hepatic tissues from HFD-fed WT and Ythdf1-KO mice. n = 6. e The level of ALT, AST, hepatic TG, TC and TBIL of HFD-fed WT and Ythdf1-KO mice was measured for 16 weeks. n = 6, respectively. f Schematic diagram of the dietary feeding scheme. We randomly injected 4-week-old Ythdf1-KO mice with AAV-8 overexpressing YTHDF1 (AAV-YTHDF1) or control vector (AAV-Vector) through the caudal vein (n = 5); they were then fed an HFD for 24 weeks. g Representative images of H&E staining and Oil Red O staining. h Quantification of the MASH scores of hepatic tissues from HFD-fed YTHDF1 rescue expression in Ythdf1-KO mice. n = 5. Quantification of the Oil Red O area of hepatic tissues from HFD-fed YTHDF1 rescue expression in Ythdf1-KO mice. n = 5. The level of serum FFA, ALT, TC and hepatic TG of HFD-fed YTHDF1 rescue expression Ythdf1-KO mice. n = 5. n.s. no significance, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001 (unpaired t-test).