Fig. 7: Methylation of the YTHDF1 protein decreases its stability. | Experimental & Molecular Medicine

Fig. 7: Methylation of the YTHDF1 protein decreases its stability.

From: Noncanonical function of epigenetic reader YTHDF1 inhibits MASLD progression by maintaining peroxisomes and mitochondrial homeostasis

Fig. 7: Methylation of the YTHDF1 protein decreases its stability.

a SILAC–MS/MS results showing that the K191 site was monomethylated in YTHDF1. b Co-IP was conducted with HepG2 and Hep3B whole-cell extracts to confirm the methylation of YTHDF1. c Co-IP results showing the methylation level of YTHDF1 under high lipid stress conditions. d Co-IP results showing that YTHDF1 K191R prevented methylation. e Western blot analysis of exogenously expressed YTHDF1-K191R mutants and endogenously expressed YTHDF1. The quantitative data of strip grayscale values are displayed on the right. f Western blot analysis of exogenously and endogenously expressed YTHDF1. The quantitative data of strip grayscale values are displayed on the right. The data are presented as the means ± s.e.m. n.s. no significance and *P < 0.05 (unpaired t-test).

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