Fig. 2: Hepatic ESRRA is required for mediating lactate clearance and gluconeogenesis in vivo. | Experimental & Molecular Medicine

Fig. 2: Hepatic ESRRA is required for mediating lactate clearance and gluconeogenesis in vivo.

From: Hepatocyte estrogen-related receptor α modulates a gluconeogenic–epigenetic crosstalk counteracting MASLD/MASH progression

Fig. 2: Hepatic ESRRA is required for mediating lactate clearance and gluconeogenesis in vivo.The alternative text for this image may have been generated using AI.

a,b An LTT was performed in 8-week-old Esrrafl/fl and EsrraHKO male mice with intraperitoneal injections with Lac (2 mg/g body weight). Blood lactate (a) and glucose (b) was measured serially, and the area under the curve (AUC) was calculated, respectively. n = 6 mice. c,d LTT comparing Esrrafl/fl and EsrraHKO mice after 12 weeks of HFD feeding. Blood lactate (c) and glucose levels (d) were examined serially, and the corresponding AUC was calculated. n = 8 mice. e Schematic diagram illustrating the experimental design for lactic acidosis assay. C57BL/6 male mice received intraperitoneal injections of LA (0.5 mg/g body weight) after treatment with either Ad-GFP or Ad-ESRRA for 5 days. f,g Blood lactate (f) and glucose (g) were measured at the indicated time and an AUC was also analyzed, respectively. n = 8 mice. h Survival curves were analyzed within 5 days following the challenge with LA. n = 8 mice. Results were expressed as mean ± s.d. Statistical analysis was performed using a two-tailed Student’s t-test (a to d, f and g) and log-rank test (h). *P < 0.05, **P < 0.01 and ***P < 0.001. Schematic in e created in BioRender. Tongling, H. (2026) https://BioRender.com/dr6ndef.

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