Fig. 4: TCTP overexpression activates BAT thermogenesis and enhances resistance to cold in NCD-fed mice. | International Journal of Obesity

Fig. 4: TCTP overexpression activates BAT thermogenesis and enhances resistance to cold in NCD-fed mice.

From: Overexpression of translationally controlled tumor protein ameliorates metabolic imbalance and increases energy expenditure in mice

Fig. 4

A Rectal temperature of 10-week-old WT and TCTP TG (\(n = 8\)). B Plasma levels of catecolamine (\(n = 6 - 7\)). C Immunoblots of ADRB3, PGC1α, UCP1 and TCTP in IBAT. D Quantification of expression levels of immunoblotted proteins (\(n = 6\)). E H&E staining and UCP1-IHC of IBAT and quantification of UCP1 stained area (%) (\(n = 5 - 6\)). The images were magnified by 10X and scale bar is 100 μm. F Changes in rectal temperature during 6 h of cold exposure at 4 °C. G The body weights of WT and TCTP TG. The body weights were measured 12 h after the cold exposure. Normalized weights of (H) liver, EpiWAT, IngWAT, and IBAT (\(n = 5 - 6\)). I Immunoblots of UCP1 and TCTP in IBAT. J Quantification of normalized protein expression levels of immunoblot in I (\(n = 6\)). Data are mean ± SEM. Unpaired t-test; *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page