Fig. 2: Changes in peripheral blood mononuclear cell and adipose tissue T-lymphocyte abundance and activation before and after 3 days of 50% calorie restriction.

A Relative proportions of CD4+ and CD8+ events within total CD3+ events before and after 3 days of 50% calorie restriction in PBMCs. B Relative proportions of CD4+ CD25+ and CD8+ CD25+ events within total CD3+ events before and after 3 days of 50% calorie restriction in PBMCs. C Relative proportions of CD4+ CD69+ and CD8+ CD69+ events within total CD3+ events before and after 3-days of 50% calorie restriction in PBMCs. D CD25 MFI on CD4+ CD25+ and CD8+ CD25 + T-lymphocytes before and after 3-days of 50% calorie restriction in PBMCs. E CD69 MFI on CD4+ CD25+ and CD8+ CD25 + T-lymphocytes before and after 3-days of 50% calorie restriction in PBMCs. F Relative proportions of CD4+ and CD8+ events within total CD3+ events before and after 3-days of 50% calorie restriction in adipose tissue. G Relative proportions of CD4 + CD25+ and CD8 + CD25+ events within total CD3+ events before and after 3 days of 50% calorie restriction in adipose tissue. H Relative proportions of CD4 + CD69+ and CD8 + CD69+ events within total CD3+ events before and after 3-days of 50% calorie restriction in adipose tissue. I CD25 MFI on CD4+ CD25+ and CD8+ CD25+ T-lymphocytes before and after 3-days of 50% calorie restriction in adipose tissue. J CD69 MFI on CD4+ CD25+ and CD8+ CD25-+ T-lymphocytes before and after 3-days of 50% calorie restriction in adipose tissue. Data are presented as group means ± SEM at each time point with individual responses overlaid. All data represent n = 12. Data were analysed using paired t-tests, or non-parametric equivalents where data were non-normally distributed (Shapiro–Wilks p > 0.05). MFI median fluorescence intensity, PBMCs peripheral blood mononuclear cells.