Fig. 3
From: TGF-β2 downregulates osteogenesis under inflammatory conditions in dental follicle stem cells
Gene profiling by RT-PCR. a The levels of the pro-inflammatory cytokines IL-6, IL-8 and IL-1β in DFSCs isolated from inflamed tissue were measured. b From the set of proteins with Mascot scores greater than 30 selected from LC-MS/MS analysis of normal and inflamed DFSCs, proteins related to osteogenic differentiation were selected. The level of expression of the genes encoding these proteins in normal and inflamed DFSCs during osteogenesis was determined by RT-PCR using specific primers. Differences in TGF-β2 gene expression in normal and inflamed DFSCs during osteogenesis were detected
