Table 1 Mass spectrometry analysis by comparison of normal and inflamed dental follicle in different bands of 1D-SDS-PAGE gel

From: TGF-β2 downregulates osteogenesis under inflammatory conditions in dental follicle stem cells

Sample

Number

NCBI BLAST

Protein name

MASCOT score

Mass/Da

Normal DF

1

GI:5821385

MTH1a (p26) (Homo sapiens)

95

22,537

 

2

GI:82571735

PPIF protein (Homo sapiens)

71

16,530

 

3

GI:284164

Arginine-rich protein—human

63

26,889

 

4

GI:5031851

Stathmin isoform a (Homo sapiens)

59

17,292

 

5

GI:223480

Dismutase, Cu/Zn superoxide

53

15,792

 

6

GI:18490199

TWF1 protein (Homo sapiens)

50

28,805

 

7

GI:385719190

Layilin isoform 3 (Homo sapiens)

38

25,555

 

8

GI:5453678

Epididymal secretory protein E1 precursor (Homo sapiens)

38

16,559

 

9

GI:30506

Desmoglein type 1 [Homo sapiens)

32

113,644

 

10

GI:9502027

Nucleotide binding protein (Homo sapiens)

27

30,204

 

11

GI:57997547

Hypothetical protein (Homo sapiens)

37

13,865

 

12

GI:3318841

Chain A, Horf6 A novel human peroxidase enzyme

34

25,011

 

13

GI:4503987

Gamma-glutamyl hydrolase precursor (Homo sapiens)

77

35,941

 

14

GI:31291

Unnamed protein product (Homo sapiens)

70

38,589

 

15

GI:34234

Laminin-binding protein (Homo sapiens)

55

31,774

 

16

GI:16549132

Unnamed protein product (Homo sapiens)

54

60,904

 

17

GI:6912682

Spondin-2 precursor (Homo sapiens)

40

35,750

 

18

GI:49256867

RAD50 protein, partial (Homo sapiens)

29

84,003

 

19

GI:18376667

hSSH-2A (Homo sapiens)

27

21,650

Inflamed DF

1

GI:36038

rho GDP dissociation inhibitor (GDI) (Homo sapiens)

54

23,179

 

2

GI:4808278

lanosterol synthase (Homo sapiens)

33

83,225

 

3

GI:4506193

Proteasome subunit beta type-1 (Homo sapiens)

137

26,472

 

4

GI:4506181

Proteasome subunit alpha type-2 (Homo sapiens)

89

25,882

 

5

GI:21465651

Chain J, Crystal structure of the mammalian 20 s proteasome at 2.75A resolution

84

22,915

 

6

GI:31543380

Protein DJ-1 (Homo sapiens)

81

19,878

 

7

GI:4506243

Polypyrimidine tract-binding protein 1 isoform a (Homo sapiens)

79

59,596

 

8

GI:4504447

Heterogeneous nuclear ribonucleoproteins A2/B1 isoform A2 (Homo sapiens)

69

35,984

 

9

GI:348239

Unnamed protein product (Homo sapiens)

50

54,233

 

10

GI:178775

Proapolipoprotein, partial (Homo sapiens)

44

28,944

 

11

GI:179462

N-acetyl-beta-glucosaminidase prepro-polypeptide, partial (Homo sapiens)

44

64,321

 

12

GI:31189

Unnamed protein product (Homo sapiens)

36

23,182

 

13

GI:182399

Farnesyl pyrophosphate synthetase (EC 2.5.1.1) (Hoo sapiens)

64

40,495

 

14

GI:557563

Transforming growth factor beta 2 (Homo sapiens)

41

47,603

 

15

GI:3294548

Cathepsin Z precursor (Homo sapiens)

40

33,860

 

16

GI:33431109

Transforming growth factor beta 1 (Homo sapiens)

39

12908

 

17

GI:10798804

Sperm antigen (Homo sapiens)

29

84,869

  1. Proteins identified by LC-MS/MS (liquid chromatography-coupled electrospray ionisation MS/MS) were searched against the NCBI database using the MASCOT search software. The list includes all significant hits