Table 1 sgRNA sets designed to target the PTCH1 locus

From: Effect of the sonic hedgehog inhibitor GDC-0449 on an in vitro isogenic cellular model simulating odontogenic keratocysts

 

sgRNA

Sequence (5′- 3′)

Extra base

Target sequence length

PTCH1-sgRNA1

Target sequence

CACTTGGGGACTATACACCCAGG

1

21 bp + PAM

PTCH1-sgRNA1-up

caccGCACTTGGGGACTATACACCC

PTCH1-sgRNA1-dn

aaacGGGTGTATAGTCCCCAAGTGC

PTCH1-sgRNA2

Target sequence

CTGGGTGTATAGTCCCCAAGTGG

1

21 bp + PAM

PTCH1-sgRNA2-up

caccGCTGGGTGTATAGTCCCCAAG

 

PTCH1-sgRNA2-dn

aaacCTTGGGGACTATACACCCAGC

PTCH1-sgRNA3

Target sequence

GGATGACGCTGAGCCACTTGGGG

0

20 bp + PAM

PTCH1-sgRNA3-up

caccGGATGACGCTGAGCCACTTG

PTCH1-sgRNA3-dn

aaacCAAGTGGCTCAGCGTCATCC

PTCH1-sgRNA4

Target sequence

GTGTCACACAGCACGTTGGGGG

0

20 bp + PAM

PTCH1--sgRNA4-up

caccGTGTCACACAGCACGTTGG

PTCH1--sgRNA4-dn

aaacCCAACGTGCTGTGTGACAC

PTCH1-sgRNA5

Target sequence

GTTTTGTGTCACACAGCACGTTGG

0

20 bp + PAM

PTCH1-sgRNA5-up

caccGTTTTGTGTCACACAGCACGT

PTCH1-sgRNA5-dn

aaacACGTGCTGTGTGACACAAAAC

  1. Underlined capital letters represent protospacer adjacent motifs (PAM) in the target sequence. Italic lowercase letters represent the sticky ends after annealing. Bold capital “G/C” represents an additional “G/C” prepended for the expression of sgRNAs from a U6 promoter
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