Fig. 1 | International Journal of Oral Science

Fig. 1

From: Prognostic value of tertiary lymphoid structure and tumour infiltrating lymphocytes in oral squamous cell carcinoma

Fig. 1

TLSs are highly organized structures in OSCC tissues and appear as clusters of B-cell follicles surrounded by T-cell zones and HEVs. a Mature TLS, similar to lymph node; CD20+ B cells form a follicular structure. The CD3+ T-cell zone is located near the follicle, and HEV are diffusely distributed around the follicle. b Immature TLS; CD20+ B cells do not form any follicular structure within the PNAd+ HEV area. CD3+ T cells were more diffusely distributed than mature TLSs. c Representative multiplex IHC images showing immune cells of immature TLS and mature TLS samples by simultaneous staining of B cells (CD20, yellow), T cells (CD3, red), mature dendritic cells (DC-LAMP, green), high endothelial venules (PNAd, cyan) and the nuclear stain DAPI (blue) (×200, original magnification). d TLS grading in OSCC tissues. Grade 0, no TLS present; grade 1, immature TLS; grade 2, mature TLS. Scale bar, 500 μm in “4X” pictures and 200 μm in “10X” pictures

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