Fig. 4
From: Novel MSX1 variants identified in families with nonsyndromic oligodontia
The expression and subcellular localization of wild-type and mutated MSX1 proteins. a Western blot analysis of total protein from wild-type or mutated MSX1 cells transfected into 293T cells using anti-GFP and anti-β-actin antibodies. Mutated proteins with normal length and truncated proteins were detected. An empty vector was transfected into cells as a negative control. b–h Subcellular localization of wild-type or mutated MSX1 in vitro. Cells transfected with GFP-G122* show expression in the entire cytoplasm with weak expression in the nucleus. GFP-A93Rfs*67 is detected in the nucleus with dense clumping. GFP-S270L is located in the same location as the wild type. GFP-Q221P and GFP-R224C show densely clumped distributions within the nucleus. The empty vector with GFP was transfected into 293T cells as a negative control. b’–h’ Nuclei stanning by DAPI, 4,6-diamino-2-phenylindole. b”-h” Merge of GFP and DAPI. MSX1 (GFP, green); nuclei (DAPI, blue)
