Fig. 6
From: Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration
The modification of chemical groups on the surface of scaffolds influence cell adhesion and osteogenic differentiation. a Fouries transform infrared microspectroscopy spectra of different scaffolds: (1) non-nitrided scaffold before soaking, (2) non-nitrided scaffold after 1 day in simulated body fluid, (3) non-nitrided scaffold after 7 day in simulated body fluid, (4) nitrided scaffold before soaking, (5) nitrided scaffold after 1 day in simulated body fluid, (6) nitrided scaffold after 7 day in simulated body fluid. b SEM images of the nitrided glass scaffold: (i) the surface topography and the internal pores from right to left; (ii) the spherical HA structures on the surface of scaffold, (iii) the spherical HA structures formed by hydroxyl-apatite needles. c Hoescht nuclear staining images of osteoblastic cells on bioglasses, non-nitrided and nitrided glass scaffolds on day 1, day 2, day 3 and day 7. d Alkaline phosphatase staining of osteoblastic cells on bioglass scaffolds and nitrided glass scaffolds. (Reused from ref. 123 with permission. Copyright 2015, Acta Materialia Inc. Published by Elsevier Ltd.)
