Fig. 2 | International Journal of Oral Science

Fig. 2

From: Copy number alteration profiling facilitates differential diagnosis between ossifying fibroma and fibrous dysplasia of the jaws

Fig. 2

Overview of the multiregional microdissection sequencing (MMS) approach. a Frozen tissue sections (10 μm-thick) were stained with hematoxylin and eosin (H&E). b Each sample contained 30–50 cells dissected by LCM from 10-µm sections to lysis buffer. c Tn5 transposase-assisted sequencing library construction. Released DNA molecules were tagmented, barcoded, and PCR amplified. d NGS reads were mapped to the reference genome and counted to infer the copy number ratio. e Samples with low quality were filtered out based on amplification noise and mapped reads. f Samples with or without CNAs were identified and used to perform downstream analysis.

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