Fig. 6 | International Journal of Oral Science

Fig. 6

From: Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity

Fig. 6

In vitro cellular performance of MCAM(+)JAG1(+) PDGFRA(−) hDPSCs. a Chondrogenic marker gene expression of SOX9, ACAN, and COL2A1 of MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 14-day culture. b Western blot and quantitative analysis of SOX9 expression in protein level after 7 days induction. c Alcian blue staining and quantification of the cultured pellets obtained from MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 28 days induction. d Adipogenic marker gene expression of PPARG, FABP4, and CEBPA of MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 14-day culture. e Western blot and quantitative analysis of PPARG expression in protein level after 14 days induction. f Oil red O staining and quantification of the adipocytes obtained from MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 28 days induction. GAPDH was used as the normalization control in qPCR. The levels of ACTIN are used as loading control in western blot. Bars represent mean ± SD values. *P < 0.05; **P < 0.01; ***P < 0.001

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