Fig. 3
Outlines of 10X spatial transcriptomics. The workflow can be divided into two stages: on the array and off the array. The works on the array consist of sample preparation, staining & imaging, permeabilization and cDNA synthesis (1–4). The works off the array include library construction, sequencing & data analysis (5–6). Whole transcriptome libraries are sequenced using Novaseq V1.5 100 cycle kits (read 1: 28 cycles, i7 index: 10 cycles, i5 index: 10, read 2: 90 cycles). Data are analyzed and visualized using Space Ranger and Loupe Browser. Loupe Browser enables direct comparison of gene expression with histology data
