Fig. 4

JMJD3 demethylates H3K27me3 in the promoter regions of NR4A1 during aberrant force-related cartilage injury. a A silence of H3K27me3 expression was presented in aberrant force-treated chondrocytes. b Staining intensity of H3K27me3 was decreased in chondrocytes upon FSS stimulation. c The volcano plot of RNA-seq was plotted to present differentially expressed genes. d The expression profiles of genes in chondrocytes with or without mechanical stress treatment. e Venn diagram analysis of RNA-seq and CUT&Tag-seq. f A comprehensive view of CUT&Tag sequence signals of H3K27me3 in the promoter regions of NR4A1. g The mRNA level of NR4A1 was greatly upregulated with FSS. h The protein expression of NR4A1 was elevated in chondrocytes exposed to FSS. i JMJD3 downregulation by adenovirus infection led to a decreased NR4A1 mRNA level. j JMJD3 downregulation suppressed the protein expression of NR4A1. k GSK-J4 administration decreased the mRNA level of NR4A1. l GSK-J4 administration inhibited the protein expression of NR4A1. m JMJD3 downregulation by adenovirus infection downregulated NR4A1 mRNA level induced by FSS treatment. n JMJD3 downregulation suppressed the protein expression of NR4A1 in FSS-treated chondrocytes. o GSK-J4 administration decreased the mRNA level of NR4A1 induced by FSS treatment. p GSK-J4 administration inhibited the protein expression of NR4A1 in FSS-treated chondrocytes. Scale bar: 50 μm *P < 0.05; **P < 0.01; ***P < 0.001