Fig. 6 | International Journal of Oral Science

Fig. 6

From: Targeted inhibition of osteoclastogenesis reveals the pathogenesis and therapeutics of bone loss under sympathetic neurostress

Fig. 6

Protection of bone mass under psychological stress is achieved by pharmacological suppression of osteoclastogenesis. a, b Micro-CT analysis of femoral bone mass and trabecular parameters. Bars: 500 μm. c, d TRAP staining for osteoclasts (black arrows indicated) and the quantification. White dashed lines indicate borders of the trabecular bone. Bars: 12.5 μm. e ELISA analysis of serum bone remodeling markers. f–h Calcein double labeling and toluidine blue images of mineralized femoral surface and osteoblasts (black arrows indicated) and the quantification. Bars: 50 μm (up) and 12.5 μm (down). n = 6 per group. RANK-Fc or clodronate was injected under the CVS modeling. Mean ± SD. *P < 0.05; NS not significant

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