Fig. 7 | International Journal of Oral Science

Fig. 7

From: Chromatin conformation of human oral epithelium can identify orofacial cleft missing functional variants

Fig. 7

Knockdown of FADS1 reduced epithelial cell migration and proliferation. a IHC staining of FADS1 in mice palatal epithelium from E12.5 to E15.5. b The relative messenger RNA expression levels of FADS1 were decreased after si-FADS1 treatment. c Western blot of FADS1 and β-actin in HIOEC treated with siRNA of negative control (NC) or FADS1. d The migration of HIOEC with treatment of si-NC or si-FADS1. Images were taken 0, 12, 24, 36, 48 h after scratch. The white dashed line represents the initial boundary of the scratch, and the red dashed line represents the anterior boundary of the migrated cells. e Immunofluorescence staining of Ki67 in HIOEC after si-FADS1 or si-NC treatment. f Quantification analysis of the relative Ki67 expression. ****P < 0.000 1. Scale bar: 100 μm

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