Fig. 2

The mandibular skeleton in Osr2-cre;Rosa26R-Fgf8 mice. a, a’, a”, b, b’, b” The lateral views of the bone and cartilage staining for the E14.5 WT (a) and Osr2-cre;Rosa26R-Fgf8 craniofacial skeleton (b). The black arrowhead in a and the red arrowhead in b pointed to the mandibular skeleton. The ventral views of the E14.5 WT (a’) and Osr2-cre;Rosa26R-Fgf8 mandibular skeleton (b’). The black arrows in a’ and b’ pointed to the terminus of Meckel’s cartilage. The lingual and buccal views of the E14.5 WT (a”) and Osr2-cre;Rosa26R-Fgf8 mandibular skeleton (b”). c, c’, c”, d, d’, d” The lateral views of the bone and cartilage staining for the E16.5 WT (c) and Osr2-cre;Rosa26R-Fgf8 craniofacial skeleton (d). The black arrowhead in c and the red arrowhead in d pointed to mandibular bone. The ventral views of the E14.5 WT (c’) and Osr2-cre;Rosa26R-Fgf8 mandibular skeleton (d’). The black arrows in c’ and d’ pointed to Meckel’s cartilage. The dashed boxes in c’ and d’ were magnified in the solid boxes, in which the black arrowhead indicated the WT Meckel’s cartilage, while the red arrowhead pointed to the extra cartilage in Osr2-cre;Rosa26R-Fgf8 Meckel’s cartilage. The lingual and buccal views of the E14.5 WT (c”) and Osr2-cre;Rosa26R-Fgf8 mandibular skeleton (d”). The red, yellow and blue arrows in c” and d” delineated the coronoid, condylar and angular processes, respectively. e, e’, e”, f, f’, f” The lateral views of the bone and cartilage staining for the E18.5 WT (e) and Osr2-cre;Rosa26R-Fgf8 craniofacial skeleton (f). The black arrowhead in e and the red arrowhead in f pointed to the mandibular skeleton. The ventral views of the E14.5 WT (e’) and Osr2-cre;Rosa26R-Fgf8 mandibular skeleton (f’). The black arrows in e’ and f’ pointed to Meckel’s cartilage. The dashed boxes in e’ and f’ were magnified in the solid boxes, in which the black arrowhead indicated the degenerated Meckel’s cartilage in WT mandible, while the red arrowhead pointed to the extra and persistent Meckel’s cartilage in Osr2-cre;Rosa26R-Fgf8 mandible. The lingual and buccal views of the E14.5 WT (e”) and Osr2-cre;Rosa26R-Fgf8 mandibular skeleton (f”). The red, yellow and blue arrows in e” and f” delineated the coronoid, condylar and angular processes, respectively. Mb Mandible, MC Meckel’s cartilage, Inc incisor. Scale bars: 2 mm