Fig. 7
From: Disrupted tenogenesis in masseter as a potential cause of micrognathia
The skeleton and masseters in the mandibles of Osr2-cre; Rosa26R-DTA and Myf5-cre; Rosa26R-DTA mice. a, a’ The gross craniofacial view (a) and the lateral view (a’) of E15.5 WT mandibular bone. b, b’ The gross craniofacial view (b) and the lateral view (b’) of the E15.5 Osr2-cre;Rosa26R-DTA mandibular bone. (The red arrowheads in a’ and b’ pointed to the coronoid and angular processes). c, c’ Masson staining showed the cross views of E15.5 WT deep (c) and superficial massters (c’). d, d’ The cross views of E15.5 Osr2-cre; Rosa26R-DTA deep (d) and superficial masseter (d’). e, e’ Myosin staining showed the E15.5 WT deep (e) and superficial masseter (e’). f, f’ Myosin staining showed the E15.5 Osr2-cre; Rosa26R-DTA deep (f) and superficial masseter (f’). The blue dotted lines circled the masseter, while the red dotted lines circled mandibular bones. The red asterisks in d, d’, f and f’ delineated the regressed myofibers of Osr2-cre; Rosa26R-DTA masseters. (Mas masseter, MC Meckel’s cartilage, Mb mandibular bone, Td masseter tendon. Scale bars: 200 μm). g, g’ The gross craniofacial view (g) and the lateral view (g’) of E16.5 WT mandibular bone. h, h’ The gross craniofacial view (h) and the lateral view (h’) of the E16.5 Myf5-cre;Rosa26R-DTA mandibular bone. (The red arrowheads in g’ and h’ pointed to the coronoid and angular processes). i, i’ Masson staining showed the cross views of E16.5 WT deep (i) and superficial masseter (i’). j, j’ The cross views of the E16.5 Myf5-cre; Rosa26R-DTA deep (j) and superficial masseter (j’). k, k’ Myosin staining showed the E16.5 WT deep (k) and superficial masseter (k’). l, l’ Myosin staining showed the E16.5 Myf5-cre; Rosa26R-DTA deep (l) and superficial masseter (l’). The red dotted boxes delineated mandibular bones. The black arrows in i, i’, k and k’ indicated the tendons of the WT masseters, while the red asterisks in j, j’, l and l’ delineated the diminished Myf5-cre; Rosa26R-DTA masseters. The red asterisks in j, j’, l and l’ delineated the diminished Myf5-cre; Rosa26R-DTA masseters. Mas masseter, MC Meckel’s cartilage, Mb Mandibular bone, Td masseter tendon. Scale bars: 200 μm
