Fig. 8
From: Disrupted tenogenesis in masseter as a potential cause of micrognathia
The markers of mechanical sensation and osteogenic differentiation in the mandibles with disabled tendons and masseters. a–f The immunostaining of Yap in E13.5 WT (a) and Osr2-cre; Rosa26R-Fgf8 mandibles (b), E13.5 WT (c) and Osr2-cre; Rosa26R-DTA mandibles (d), as well as E13.5 WT (e) and Myf5-cre; Rosa26R-DTA mandibles (f). (g, g’, h, h’, i, j) The immunostaining of p-Erk1/2 in E13.5 WT (g) and Myf5-cre; Rosa26R-DTA mandibles (h), as well as E13.5 WT (i) and Osr2-cre; Rosa26R-DTA mandibles (j). The boxed areas in g and h were amplified in g’ and h’, respectively. k, l, m, m’, n, n’ The immunostaining of p-Smad1/5/8 in E13.5 WT (k) and Osr2-cre; Rosa26R-Fgf8 mandibles (l), as well as E13.5 WT (m) and Myf5-cre; Rosa26R-DTA mandibles (n). The boxed areas in m and n were amplified in m’ and n’, respectively. o, o’, p, p’, q, r The immunostaining of Osterix in E13.5 WT (o) and Osr2-cre; Rosa26R-Fgf8 mandibles (p), as well as E13.5 WT (q) and Myf5-cre; Rosa26R-DTA mandibles (r). The boxed areas in o and p were amplified in o’ and p’, respectively. Red dashed lines encircled masseters, blue dashed lines encircled mandibular bones, and the black dashed lines encircled masseter tendons; the red arrowheads pointed to the periosteal mesenchyme of mandibular bones; Scale bars: 200 μm
