Fig. 5
From: Metformin can mitigate skeletal dysplasia caused by Pck2 deficiency
Skeleton dysplasia in OC-Cre; Pck2f/f mice were mitigated by metformin treatment. a The timetable of metformin administration for pregnant mice of indicated phenotypes. b–e HE staining of the head sections from P0 (b, d), P5 (c, e) mice with indicated phenotypes. Cranial bone width was indicated with arrows in b, c. Scale bars: 500 µm. n = 3. f, g HE staining of the femur sections from P0 (f), P5 (g) mice with indicated phenotypes. Scale bars: 500 µm. n = 3. h–j Quantitative analysis of relative mandibular length (n = 3), relative mandibular width (n = 3), and relative cranial width (n = 3). *P < 0.05, ns not significant
