Fig. 2
Deletion of RGS12 in myeloid lineage promotes the progression of oral cancer. a Diagram of the protocol to generate oral carcinogenesis model. b H&E-stained sections of tongue epithelium at 0, 8, and 16 weeks after 4NQO administration. Scale bar, 50 µm. c H&E-stained sections of tongue epithelium (around basement membrane, yellow dashed line) at 0, 8, and 16 weeks after 4NQO administration. Scale bar, 20 µm. d Immunofluorescence staining for RGS12 and F4/80 expression in 4-week and 8-week 4NQO treated tongue epithelium. Scale bar, 20 µm. e The relative intensity of RGS12 per F4/80+ cell as indicated in d. **P < 0.01, n = 5. f Diagram of the protocol for the RGS12 conditional knockout (RGS12 cKO, LysmCre; RGS12fl/fl) mice and Control (Ctrl, LysmCre) mice with 8-week 4NQO induction. g H&E staining of Ctrl and RGS12 cKO tongue epithelial layers at 8 weeks after 4NQO induction (n = 10). Scale bar, 100 µm. The bar graph showed the percentages of the indicated lesion types present in the tongues. SCC, squamous cell carcinoma; CIS, squamous carcinoma in situ
