Fig. 3

RGS12 in TAMs promotes the polarization of M1 macrophages. a, b Immunofluorescence for CD86 and CD163 expression to detect M1 and M2 macrophages in tongue epithelial layers from control and RGS12 cKO mice a. The right panels b indicate the quantitative data of relative intensity of CD86 or CD163 protein. Scale bar, 50 µm. *P < 0.05, **P < 0.01, n = 5. c, d TAMs were obtained from the oral cancer tissues of WT mice. The M1 macrophage (CD86) and M2 macrophage (CD163) were detected by immunofluorescence staining c. Scale bar, 20 μm. Statistical data showed the overexpression of RGS12 promotes M1 macrophages but inhibits M2 macrophage polarization d. **P < 0.01, ***P < 0.001, n = 5