Fig. 6

RGS12 promotes the ubiquitination and degradation of KIF2A in TAMs. a, b Western blot analysis of ubiquitin (Ub) and KIF2A expression in RGS12 cKO and control TAMs. Data are expressed as mean ± SEM (n = 3, **P < 0.01, ***P < 0.001). c, d The TAMs from WT mice were transfected with control (pCMV) and RGS12 OE (pCMV-RGS12) plasmids for 48 h. Western blot analysis of the ubiquitin (Ub) and KIF2A levels in TAMs. Data are expressed as mean ± SEM (n = 3, **P < 0.01). e Transcriptional levels of KIF2A in TAMs as described in a, d. Data are reported as means ± SEM (NS no significance, n = 5, P > 0.05)