Fig. 7 | International Journal of Oral Science

Fig. 7

From: RGS12 represses oral squamous cell carcinoma by driving M1 polarization of tumor-associated macrophages via controlling ciliary MYCBP2/KIF2A signaling

Fig. 7

RGS12 activates MYCBP2 through p-Tyr in TAMs. a Identification of RGS12 interacting proteins. b, c The top ten unique proteins b and top ten ubiquitin-related proteins c were indicated in tables. d Immunofluorescence indicates that RGS12 and MYCBP2 colocalize in TAMs (Scale bar, 10 µm). e RGS12 associates with MYCBP2 in TAMs. The cell lysates were incubated with anti-RGS12 or immunoglobulin G (IgG) antibodies and the bound MYCBP2 was detected by immunoblotting. f TAMs from oral cancer tissues of Ctrl or RGS12 cKO mice were immunoblotted with an antibody against mouse MYCBP2. (NS no significance, n = 3, P > 0.05). g The TAM lysates from oral cancer tissues of Ctrl or RGS12 cKO mice were incubated with anti-p-Tyr or IgG antibodies, and bound protein was examined by western blot with the MYCBP2, p-Tyr, and β-Actin antibodies, as indicated. Note that RGS12 cKO decreases the phosphorylation (p-Tyr) of MYCBP2. ***P < 0.001, n = 3

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