Fig. 1

Construction of the severe tooth germ injury model based on transgenic line Tg(dlx2b:Dendra2-NTR). a Expression pattern of dlx2b at a 3 dpf. Dorsal and ventral views are shown. The probe was dlx2b. The white arrows point to the probe-binding site. b Total body images of wild-type and transgenic line Tg(dlx2b:Dendra2-NTR) at 3 dpf. The white arrows point to the site of fluorescence expression, and the white “*” indicates no obvious fluorescence expression. c Embryos at 3 dpf were treated with Mtz for 48 h. d Antibody staining of Dendra2 protein (green) representing tooth germ was performed on the 3 dpf and 5 dpf embryos. e TUNEL staining was performed after 12 h of Mtz treatment. Apoptosis (red) occurred at the tooth germ (green) location. f, g In situ hybridization was performed at 3 dpf and 5 dpf intervals. Dorsal and ventral views are presented. The probes were scpp5 and fth1b, respectively. The white arrows point to the probe-binding site, and the white “*” indicates no obvious probe-binding site. The number in the lower right corner is the number of positive/total number of experiments. Scale bar for a, b, f, and g = 100 μm; scale bar for d and e = 20 μm