Fig. 3

mTORC1 signaling was activated in the early stage of tooth germ repair. a Embryos were analyzed at R6h and R24h after Mtz treatment. b Antibody staining was performed on R6h embryos, and p-4E-BP1 (red) was located by confocal laser scanning. p-4E-BP1 was co-stained with the tooth germ (green) at R6h. c Co-localization analysis of p-4E-BP1 (red) and tooth germ (green) in the Mtz-treated group at R6h. d, e The cell proliferation (red) was displayed in tooth germ (green) and surrounding anatomical position at R24h by EdU staining, and the statistical data of EdU⁺ cells were analyzed (n = 3). Scale bar for b and d = 20 μm (***P ＜ 0.001)