Fig. 4
Rapamycin inhibited the repair of severely injured tooth germs. a Embryos at 3 dpf were treated with Rapamycin and Mtz for 48 h, and then the embryos were analyzed from R0D to R2D. b Antibody staining was performed on R0–2D embryos after treatment. Green fluorescence indicated tooth germs. c Alizarin Red staining was performed on R1–2D embryos after treatment. The black arrow points to the mineralized pharyngeal teeth, and the black “*” represents no obvious mineralization. d EdU staining showed the cell proliferation (red) in the tooth germ (green) at R24h. e Statistical analysis of EdU+ cells (n = 3). Scale bar for c = 100 μm; scale bar for b and d = 20 μm (***P ＜ 0.001; ****P ＜ 0.000 1)
