Fig. 3 | International Journal of Oral Science

Fig. 3

From: Down-regulation of DNA key protein-FEN1 inhibits OSCC growth by affecting immunosuppressive phenotypes via IFN-γ/JAK/STAT-1

Fig. 3

FEN1 knockdown in Cal-27 suppresses tumor growth in vivo and cause DNA damage. a Schematic diagram of xenograft mouse models in vivo. b, c tumors were measured with calipers, and volume was calculated every 7 days, and the line chart shows the differences (n = 6). d mice weight was measured and documented every week (n = 6). e Representative immunohistochemistry staining of PCNA in tumors of mouse models. Scale bar: 200 μm. n = 6; error bars, mean ± SD; by t test. f Comet assay demonstrated that FEN1 knockdown could increase the percentage of Tail DNA (×200) Scale bar: 200 μm. g, h immunofluorescence showed FEN1 downregulation could induce DNA double strain damage, the expression of pH2AX and 53BP1 significantly increased, forming foci (×400) Scale bar: 200 μm. n = 3; error bars, mean ± SD, n.s., not significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1; by t test or two-way ANOVA

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