Fig. 4

Adsorption of HMGB1 onto the surface of BCP and activation of DCs. a ELISA of danger signal proteins in the PBS-treated BCP, LW-BCP, and MW-BCP eluents (n = 3). b ELISA of HMGB1 and HSP60 in muscles (n = 3). c IHC staining showed that HMGB1 had adsorbed onto the surface of BCP (marked by red arrows). d, e Flow cytometry and semiquantification of DC activation markers CD80 and CD86 (n = 3)