Fig. 5
From: Double heterozygous pathogenic mutations in KIF3C and ZNF513 cause hereditary gingival fibromatosis
Confirmation of the binding and regulation of ZNF513 to KIF3C. a The expression of KIF3C and ZNF513 derived from the GTEx database in 54 tissues showed that they are co-expressed and positively correlated in the brain, kidney, and other tissues. Each grey dot represents one sample. Red lines are regression equations. b, c ChIP was performed in NHGFs and patient’s gingival tumor fibroblasts by ZNF513 antibody and control antibodies, respectively. ChIP-qPCR and agarose gel electrophoresis showed that ZNF513 binds to KIF3C exon 1. N-NC, N-PC, and N-KIF3C indicate negative control, positive control, and experiment group (ZNF513 antibody), respectively, in NHGFs. P-NC, P-PC, and P-KIF3C indicate negative control, positive control, and experiment groups of the patient’s gingival tumor fibroblasts, respectively. d, e A ZNF513 knockout HGF cell line was constructed with CRISPR/Cas9, and the mRNA and protein levels of KIF3C showed a significant decrease. NC = HGF-lentiCRISPR
