Fig. 1
From: MLL1 inhibits the neurogenic potential of SCAPs by interacting with WDR5 and repressing HES1
MLL1 knock-down enhanced the expression of neural markers in SCAPs. a The MLL1 knock-down efficiency was tested by Western blot. Histone H3 served as an internal control; b, c MLL1 knock-down increased the neurosphere volume compared to the SCAP/Scramsh group. Scale bar, 100 μm; Real-time RT-PCR analysis revealed that MLL1 knock-down increased the expression of NeuroD (d), NCAM (e), and TH (f) in SCAPs. GAPDH served as an internal control; g–j The immunofluorescence staining revealed that the numbers of Nestin-positive and βIII-tubulin-positive neurospheres were significantly increased in the SCAP/MLL1sh group compared to the SCAP/Scramsh group. Scale bar, 100 μm. Statistical significance was determined using Student’s t test. All error bars represent SD. (n = 3). *P ≤ 0.05. **P ≤ 0.01
