Fig. 5
From: MLL1 inhibits the neurogenic potential of SCAPs by interacting with WDR5 and repressing HES1
WDR5 knock-down enhanced the expression of neural markers in SCAPs. a, b WDR5 knock-down increased the neurosphere volume compared to the SCAP/Scramsh group. Real-time RT-PCR analysis revealed that WDR5 knock-down increased the expression of NeuroD (c), NCAM (d), and TH (e) in SCAPs. GAPDH served as an internal control. f–i The immunofluorescence staining revealed that the numbers of Nestin-positive and βIII-tubulin-positive neurospheres were significantly increased in the SCAP/WDR5sh group compared to the SCAP/Scramsh group. Statistical significance was determined using Student’s t test. All error bars represent SD. (n = 3). *P ≤ 0.05. **P ≤ 0.01
