Fig. 6
From: MLL1 inhibits the neurogenic potential of SCAPs by interacting with WDR5 and repressing HES1
WDR5 overexpression inhibited the expression of neural markers in SCAPs. a, b WDR5 over-expression decreased the neurosphere volume compared to the Vector group. Real-time RT-PCR analysis revealed that WDR5 over-expression decreased the expression of NeuroD (c), NCAM (d), and TH (e) in SCAPs. GAPDH served as an internal control. f–i The immunofluorescence staining revealed that the numbers of Nestin-positive and βIII-tubulin-positive neurospheres were significantly decreased in the WDR5 over-expressed SCAPs compared to the SCAP/Vector group. Statistical significance was determined using Student’s t test. All error bars represent SD. (n = 3). *P ≤ 0.05. **P ≤ 0.01
