Fig. 7
From: MLL1 inhibits the neurogenic potential of SCAPs by interacting with WDR5 and repressing HES1
Knocking down MLL1 or WDR5 promotes the expression of HES1, NR4A2, and IL-6 through H3K4me3 methylation. Real-time RT-PCR analysis revealed that the expression of neural-related genes in the SCAP/Myc-WDR5 group was significantly reduced, including HES1 (a), NR4A2 (b), EGR1 (c), IL-6 (d), FOS (e), ATF3 (f), ID4 (g), and GDF15 (h). GAPDH served as an internal control. i–k Real-time RT-PCR analysis revealed that the knock-down of WDR5 or MLL1 up-regulated HES1, NR4A2, and IL-6 expression in SCAPs. GAPDH served as an internal control. l–m The ChIP assay results revealed that depletion of MLL1 or WDR5 decreased the enrichment of H3K4me3 in the HES1, NR4A2, and IL-6 promoters. Statistical significance was determined using one-way ANOVA, or the Kruskal–Wallis test. All error bars represent SD. (n = 3). *P < 0.05. **P ≤ 0.01
