Fig. 9
From: MLL1 inhibits the neurogenic potential of SCAPs by interacting with WDR5 and repressing HES1
HES1 knock-down reduced the expression of neural markers in SCAPs. a The HES1 knock-down efficiency was tested by Western blot. β-actin served as an internal control; b, c HES1 knock-down reduced the neurosphere volume compared to the SCAP/Consh group; Real-time RT-PCR analysis revealed that HES1 knock-down reduced the expression of NeuroD (d), TH (e), and βIII-tubulin (f) in SCAPs. GAPDH served as an internal control. g–j The immunofluorescence staining revealed that the numbers of Nestin-positive and βIII-tubulin-positive neurospheres were significantly decreased in the SCAP/HES1sh group compared to the SCAP/Consh group. Statistical significance was determined using Student’s t test. All error bars represent SD. (n = 3). *P ≤ 0.05. **P ≤ 0.01
