Fig. 5

Morphology of organoids grown at different media compositions. Organoids were grown from aDESCs embedded in Matrigel using media supplemented with Wnt3a, R-spondin1, and Egf (minimal), minimal + Noggin (+N), +N + Fgf10 (+NF), or +NF + dibenzazepine (+NFD) for 2 weeks. a Maximum intensity projection of serial Z-section DIC images of whole well with multiple organoids. Scale bar = 500 µm. b H&E-stained paraffin sections. In a and b, red arrows indicate hollow spheroids. Scale bar = 100 µm. c Bar plot of the fraction of solid (blue) and hollow (red) spheroids as a function of the media composition (n = 3). Data are represented as the mean ± SD. d Bright-field images of representative organoids. Scale bar = 100 µm