Fig. 4
From: Sema3A secreted by sensory nerve induces bone formation under mechanical loads
Mechanical loads promote neurons secret Sema3A. a Western Blot showed that mechanical loads promoted the expression of Sema3A in neurons. b Western Blot showed that 24-hour mechanical loads inhibited the expression of Sema3A, Runx2, and NGF in hPDLCs. c Schematic representation for exploring the efforts of the supernatant from neurons receiving 24-hour mechanical loads (conditional medium) on axon extension and hPDLCs differentiation. d 24-hour fixed-point photography showed that the conditional medium inhibited neurons growth. e Western Blot showed the conditional medium promoted the expression of Runx2 in hPDLCs. f Quantification of (e) (n = 3). mean ± SD, and Two-tailed Student’s t-test was used for comparison. ***P < 0.001
