Fig. 3
From: Transcriptomic and cellular decoding of scaffolds-induced suture mesenchyme regeneration
Transcriptome analysis of scaffold-mediated MSC recruitment. a Workflow of the RNA-Seq experiment with three major steps: nascent tissue isolation, Cd45- cell sorting (Gating strategy of FCM for Cd45- cells), and RNA-seq analysis (PCA, Principal component analysis). b Heatmap analysis of DEGs. c Volcano plot illustrating the DEGs in PLA compared with NC, GelMA, and CTS, respectively. The upregulated DEGs and other genes of interest are labeled. d The top 20 up-enrichment GO terms of cellular component and molecular function in the PLA group compared with NC, GelMA, and CTS groups. e Venn diagram displaying common upregulated DEGs in chemokine/cytokine activity (GO:0008009 and GO:0005125) of the three comparisons (PLA-vs-NC, PLA-vs-GelMA, and PLA-vs-CTS). f The top 15 up-enrichment KEGG pathways in the PLA group compared with NC, GelMA, and CTS groups. g Venn diagram illustrating common upregulated DEGs in cytokine-cytokine receptor interaction (rno04060) of the three comparisons (PLA-vs-NC, PLA-vs-GelMA, and PLA-vs-CTS). NC, suture-bony composite defects without scaffold implantation
