Fig. 6

JPX upregulates the expression level of PLAU by inhibiting miR-193b-3p. a DEGs genes downstream of miR-193b-3p include PLAU, LAMC1, STMN1, and TGFBR3. b CCK-8 assay of CAL27 cells with JPX/miR-193b-3p knockdown or overexpression. c The relative expression levels of PLAU, LAMC1, STMN1, and TGFBR3 in CAL27 cells with JPX/miR-193b-3p knockdown or overexpression. d Immunofluorescence images for the localization and the expression of PLAU in CAL27 cells with JPX/miR-193b-3p knockdown or overexpression. 40×, scale bar = 50 μm. e Protein expression and mRNA expression of RRM2 based on CPTAC database. f Expression of PLAU is shown in UMAP of epithelial cell populations in HNSCC. g Available pathology images of PLAU localization and expression in HC and HNSCC were obtained from the HPA database. h Different expression levels of PLAU between the HC and HNSCC groups. Con control group, neg empty vector group, pos positive control group, L- JPX knockdown group, V. vehicle group, L+ JPX overexpressed group, NCM- miR-193b-3p inhibitor empty plasmid group, NCM+ miR-193b-3p mimic empty plasmid group, M- miR-193b-3p knockdown group, M+ miR-193b-3p overexpressed group, L+M+ JPX overexpressed and miR-193b-3p overexpressed group. Wilcoxon signed-rank test: *P < 0.05, **P < 0.01, and ***P < 0.001