Table 3 Role of H3K27 histone methylation modification in Osteo-/Odontogenic differentiation of bone/dental-derived MSCs
Epigenetic modification factors | Epigenetic marker | Cell | Function | Reference | |
|---|---|---|---|---|---|
Normal | EZH2 | H3K27me3↑ | hDPCs | EZH2 inhibits the osteogenic differentiation and mineralization of hDPCs through the β-catenin pathway and promotes their proliferation | |
H3K27me3↑ | PDLSCs | LncRNA SNHG1 increases the level of H3K27me3 at the KLF2 promoter through the action of EZH2, thereby repressing the expression of ALP, Osx, and OCN | |||
H3K27me3↑ | PDLSCs | LncRNAs SNHG8 and EZH2 reciprocally regulate each other to silence KLF transcription and inhibit osteogenic differentiation | |||
H3K27me3↑ | hDPCs | LncRNA CARMN promotes odontogenic differentiation of hDPCs by inhibiting the activity of EZH2 | |||
EZH2 KDM6A | H3K27me3 | BM-MSCs | EZH2 and KDM6A co-regulate the expression of H3K27me3 in the promoter region of genes, thereby influencing the expression of Runx2, OPN, and OCN | ||
EZH2 KDM6A/B | H3K27me3 | hDFSCs | LncRNA HOTAIRM1 promotes DFSCs-mediated bone regeneration by regulating the HIF1α/KDM6/EZH2/H3K27me3 axis | ||
KDM6B | H3K27me3↓ | hDPCs | The transcription of downstream odontogenic marker genes, including OSX, BGLAP, and SPP1, is initiated | ||
KDM6B | H3K27me3↓ | hDPSCs | The miR-93-5p targets KDM6B and removes the H3K27me3 marker on the BMP2 promoter, thereby regulating the odontogenic differentiation and dentin formation of DPSCs | ||
Inflammatory conditions | EZH2 | H3K27me3↑ | PDLSCs | Knockdown of EZH2 promotes osteogenic differentiation by inhibiting the TLR4/MyD88/NF-κB signaling pathway | |
KDM6B | H3K27me3↓ | PDLSCs | KDM6B forms a protein complex with BCRO to inhibit IGFBP5 transcription. KDM6B removes H3K27me3 from the promoter region of the downstream gene IGFBP5 and mediates periodontal tissue regeneration | ||
