Fig. 5
From: Physiologically relevant coculture model for oral microbial-host interactions
The asymmetric coculture system further enables oxygen-sensitive anaerobe Pg to proliferate normally in coculture with TIGK cell monolayers. a CFUs of Pg 33277 in the apical chamber under normoxic and asymmetric coculture conditions at 0, 24, and 48 h time points (n = 2, N = 3). The MOI was 1. b Representative flow cytometry results for TIGKs after coculture with Pg 33277, as assessed by the SYTOX green staining. c Quantification of TIGK viability after asymmetric coculture with Pg 33277 based on the above flow cytometry results (*0.01 < P < 0.05, N.S., P > 0.05, n = 2, N = 3)
