Fig. 8

SMAD7 binds to β-CATENIN to form a transcriptional complex in hDPSC. a STRING database prediction showing potential interaction between SMAD7 and β-CATENIN. b Co-IP assay showing the interaction between SMAD7 and β-CATENIN. c Immunofluorescent co-staining for SMAD7, β-CATENIN, and LEF1 in hDPSCs. Scale bar: 30 μm. d Yeast two-hybrid assay demonstrating direct interactions among SMAD7, p-SMAD2/3, and β-CATENIN. Positive colonies are indicated by red spots