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Profile of gut flora in hypertensive patients with insufficient sleep duration

Journal of Human Hypertension volume 36, pages 390–404 (2022)Cite this article

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Abstract

Recently, the contribution of both insufficient sleep duration and gut microbiome dysbiosis to hypertension (HTN) have been revealed, yet the profile of gut flora in hypertensive patients with insufficient sleep duration remains unknown. To examine this condition, the specific shifts in the fecal microbiome of 53 participants with or without HTN were investigated. The patients were divided into those who slept short (≤6 h) or optimal (6–9 h) duration per day. Comprehensive metagenomic sequencing analysis of fecal specimens was performed in healthy controls with sufficient sleep (s-CTR, n = 10), healthy controls with insufficient sleep (ins-CTR, n = 6), hypertensive patients with sufficient sleep (s-HTN, n = 25), and HTNs complicated by short sleep duration (ins-HTN, n = 12). We found that the α-diversity and β-diversity were quite similar between s-HTN and ins-HTN. Similarities were also observed in the enterotype distribution between s-HTN and ins-HTN subjects. In addition, the enrichment of gut bacteria was evident, such as Fusobacterium mortiferum and Roseburia inulinivorans in ins-HTN subjects. Several functional modules that were distinct between s-HTN and ins-HTN subjects were identified, which were unique to hypertensive patients with insufficient sleep duration. Overall, the data demonstrated that the gut microbial features were largely maintained in hypertensive participants with insufficient sleep duration.

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Fig. 1: Gut microbial diversity between hypertensive patients with and without sufficient sleep duration.
Fig. 2: Enterotype profile of s-HTN and ins-HTN.
Fig. 3: Taxonomic annotation and abundance profile of the study cohort.
Fig. 4: Differential genera between hypertensive patients with sufficient and insufficient sleep duration.
Fig. 5: Bacterial species exhibit distinct hypertensive patients with short sleep duration from those with sufficient sleep.
Fig. 6: Profile of microbial functions in hypertensive patients with sufficient or short sleep duration.

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Funding

This work was supported by the National Natural Science Foundation of China (81670214, 81870308, and 81970271), the Natural Science Foundation of Beijing Municipality (7172080, 7204242), the Capital Medical University Research and Development Fund (1200020102), and the National Nature Cultivation Fund of Beijing Chaoyang Hospital (CHPY202050).

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  1. These authors contributed equally: Jie Jiao, Ying Dong

Authors and Affiliations

  1. Heart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China

    Jie Jiao, Ying Dong, Pan Wang, Kun Zuo, Chunming Han, Jiuchang Zhong, Xinchun Yang & Jing Li

  2. State Key Laboratory of Cardiovascular Disease of China, Hypertension Center, National Center for Cardiovascular Diseases of China, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

    Jun Cai

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Contributions

JL, XY, and JJ conceived the study, directed the project, designed the experiments. JL, JJ, and YD interpreted the results, and wrote the manuscript. PW, KZ, CH, JC, and JZ recruited and collected the clinical details from the subjects. JJ, YD, and JL analyzed the data. XY, JJ, YD, and JL revised the manuscript. JJ and YD contributed equally to this work and are jointly first authors.

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Correspondence to Xinchun Yang or Jing Li.

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Jiao, J., Dong, Y., Wang, P. et al. Profile of gut flora in hypertensive patients with insufficient sleep duration. J Hum Hypertens 36, 390–404 (2022). https://doi.org/10.1038/s41371-021-00529-0

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