Fig. 7 | Laboratory Investigation

Fig. 7

From: TSG-6 secreted by bone marrow mesenchymal stem cells attenuates intervertebral disc degeneration by inhibiting the TLR2/NF-κB signaling pathway

Fig. 7

BMSCs and TSG-6 downregulated the TLR2/NF-κB signaling pathway and reduced the protein levels of MMPs and inflammatory cytokines in degenerated NP tissue. The protein levels of TLR2, MyD88, p-p65, p65, MMP-3, and MMP-13 in NP tissue were measured by western blotting. a, b Representative image of protein levels of TLR2, MyD88, p-p65, p65, MMP-3, and MMP-13. c–h Quantitative analysis of western blotting results showed that BMSC transplantation and TSG-6 injection significantly inhibited the activation of the TLR2/NF-κB signaling pathway and reduced the expression of MMPs in degenerated NP tissue. BMSC transplantation and TSG-6 injection significantly reduced the protein levels of i IL-6 and j TNF-α in degenerated NP tissue. The data are expressed as the means ± SD (n = 6 in each group). ##P < 0.01 versus the control group. **P < 0.01 versus the surgery group

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