Fig. 4 | Laboratory Investigation

Fig. 4

From: Lack of hepatic stimulator substance expression promotes hepatocellular carcinoma metastasis partly through ERK-activated epithelial–mesenchymal transition

Fig. 4

EMT marker expression in HSS-Tx or HSS-shRNA HCC cells. a, b The expression of EMT markers in HSS-Tx and HSS-shRNA cells (HepG2 cells). Western blot analysis was performed using antibodies against the epithelial markers, E-cadherin and ZO-1, and the mesenchymal markers, α-SMA and Fibronectin, respectively. The bands were analyzed with Image J software and the relative densities of the bands were normalized to GAPDH. The values are expressed as the means ± SD of three independent experiments. c, d The IHC assay was conducted to detect the expressions of HSS, β-catenin, and E-cadherin in the tumors of MHCC97H cells. Tissue sections are representative of at least five samples in each group. For each section, at least six fields were randomly chosen for histological analysis. Quantitation of positive area was analyzed with Image J software. *P < 0.05, **P < 0.01, and ***P < 0. 001.

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