Table 5 Concordance between RT-qPCR and IHC/FISH for PGR in IDCA cohort

From: Macrodissection prior to closed system RT-qPCR is not necessary for estrogen receptor and HER2 concordance with IHC/FISH in breast cancer

 

PR positive

PR negative

Total

Non m-d samples (P  <  0.001; sensitivity = 0.86; specificity = 0.88; PPV =  0.95; NPV = 0.71)

 dCt ≥ −3.5

37

2

39

 dCt < −3.5

6

15

21

 Total

43

17

60

m-d samples (P < 0.001; sensitivity = 0.84; specificity = 1; PPV = 1; NPV = 0.71)

 dCt ≥ −3.5

36

0

36

 dCt < −3.5

7

17

24

 Total

43

17

60